the evaluation of genetic diversity of einkorn wheats using microsatellite markers

نویسندگان

هادی خارستانی

دانش آموخته کارشناسی ارشد گروه زراعت و اصلاح نباتات، دانشگاه ایلام علی اصغر نصراله نژاد قمی

استادیار گروه زراعت و اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی گرگان علی اشرف مهرابی

استادیار گروه زراعت و اصلاح نباتات، دانشگاه ایلام

چکیده

genetic diversity in 22 accessions of einkorn wheat including thirteen t. boeoticum, four t. monococcum and five t.urartu, evaluated by 24 microsatellite markers originated from a genome of bread wheat. twenty of these ssrs amplified 86 polymorphic fragments among accessions. allele number per locus ranged from 2 to 9 and 4.1 in average. polymorphism information content was calculated from 0.09 (xgwm99-1a) to 0.86 (xgwm165-4a). dendrogram constructed on dice dissimilarity coefficients by neighbor-joining algorithm separated accessions in three distinct groups. t. monococcum with two of t. urartu accessions made group i. but t. boeoticum and t. urartu species join together could not be separated and scattered in the rest groups, also these two einkorn wheat species have not any genetically and geographically differentiation. the collected accessions from northwestern regions of iran have too much more diversity than the western regions of country that we could use from this diversity for found resistant genes about biotic (pest) and abiotic (drought and salt) stress.

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